Recombinant Human IL-10 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1064-ILB

Analyzed by SEC-MALS.
R&D Systems, part of Bio-Techne
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1064-ILB-010
1064-ILB-01M
1064-ILB-050
1064-ILB-250
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Key Product Details

Source

E. coli

Accession #

P22301.1

Structure / Form

Noncovalently-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all IL-10 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human IL-10 protein
Ser19-Asn178, with a N-terminal Met

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

19 kDa

SDS-PAGE

18 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507. The ED50 for this effect is 0.075-0.750 ng/mL.

Scientific Data Images for Recombinant Human IL-10 Protein, CF

Recombinant Human IL‑10 Protein SEC-MALS.

Recombinant human IL-10 (Catalog # 1064-ILB) has a molecular weight (MW) of 36.7 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Recombinant Human IL‑10 Protein Bioactivity.

Measured in a cell proliferation assay using MC/9‑2 mouse mast cells. The ED50 for this effect is 0.075-0.750 ng/mL.

Recombinant Human IL‑10 Protein SDS-PAGE.

2 μg/lane of Recombinant Human IL‑10 Protein (Catalog # 1064-ILB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18.4 kDa.

Formulation, Preparation and Storage

1064-ILB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 100-500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL‑10 family of alpha‑helical cytokines that also includes IL‑19, IL-20, IL‑22, IL‑24, and IL‑26/AK155 (1, 2). IL‑10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2-5). Mature human IL-10 shares 72%-86% amino acid sequence identity with bovine, canine, equine, feline, mouse, ovine, porcine, and rat IL‑10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL‑10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (6, 8, 9). The IL-10 dimer binds to two IL‑10 R alpha/IL‑10 R1 chains, resulting in recruitment of two IL‑10 R beta/IL‑10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (10). IL-10 R beta does not bind IL‑10 by itself but is required for signal transduction (1). IL‑10 R beta also associates with IL-20 R alpha, IL‑22 R alpha, or IL‑28 R alpha to form the receptor complexes for IL‑22, IL‑26, IL‑28, and IL‑29 (11-13). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (14-16). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References

  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. Sabat, R. et al. (2010) Cytokine Growth Factor Rev. 21:331.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  9. Syto, R. et al. (1998) Biochemistry 37:16943.
  10. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  11. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  12. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  13. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  14. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  15. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  16. Blackburn, S.D. and E.J. Wherry (2007)Trends Microbiol. 15:143.

Long Name

Interleukin 10

Alternate Names

CSIF, GVHDS, IL10, IL10A, TGIF

Entrez Gene IDs

3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)

Gene Symbol

IL10

UniProt

P22301.1

Additional IL-10 Products

Product Documents for Recombinant Human IL-10 Protein, CF

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Product Specific Notices for Recombinant Human IL-10 Protein, CF

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